Composite

Part:BBa_K1711001:Design

Designed by: Anastasia Nicolov, Tom Duan, Justin Jenkins   Group: iGEM15_Washington   (2015-09-18)


yeVenus-TheoA; coding sequence for enhanced yellow fluorescent protein and theophylline-sensative ap


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

We had to choose a linker sequence between the Venus and the aptazyme that would be free of secondary structure as a RNA transcript.


Source

yeVenus is a modified yellow fluorescent protein, originating from the genome of A. victoria. The theophylline-repsonsive aptamer sequence originates from the genome of S. cerevisiae. Coding sequence for yellow fluorescence protein was given to us by Klavins lab at University of Washington. The aptazyme sequnce was given to us by the Smolke lab at Stanford.[1]

[1]Townshend B, Kennedy AB, Xiang JS, Smolke CD. High-throughput cellular RNA device engineering. Nature Methods, Jun 2015. doi:10.1038/nmeth.3486

References